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Science 2 September 1994:
Vol. 265. no. 5177, pp. 1448 - 1451
DOI: 10.1126/science.8073288
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Articles

Science, Vol 265, Issue 5177, 1448-1451
Copyright © 1994 by American Association for the Advancement of Science

articles

Engineering poliovirus as a vaccine vector for the expression of diverse antigens

R Andino, D Silvera, SD Suggett, PL Achacoso, CJ Miller, D Baltimore, and MB Feinberg

Department of Microbiology and Immunology, University of California, San Francisco.

As a step toward developing poliovirus as a vaccine vector, poliovirus recombinants were constructed by fusing exogenous peptides (up to 400 amino acids) and an artificial cleavage site for viral protease 3Cpro to the amino terminus of the viral polyprotein. Viral replication proceeded normally. An extended polyprotein was produced in infected cells and proteolytically processed into the complete array of viral proteins plus the foreign peptide, which was excluded from mature virions. The recombinants retained exogenous sequences through successive rounds of replication in culture and in vivo. Infection of animals with recombinants elicited a humoral immune response to the foreign peptides.


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