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Science 20 October 1995:
Vol. 270. no. 5235, pp. 480 - 483
DOI: 10.1126/science.270.5235.480
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Reports

Physical Map and Organization of Arabidopsis thaliana Chromosome 4

Renate Schmidt (1),  Joanne West,  Karina Love,  Zoë Lenehan,  Clare Lister,  Helen Thompson,  David Bouchez,  Caroline Dean (2)

A physical map of Arabidopsis thaliana chromosome 4 was constructed in yeast artificial chromosome clones and used to analyze the organization of the chromosome. Mapping of the nucleolar organizing region and the centromere integrated the physical and cytogenetic maps. Detailed comparison of physical with genetic distances showed that the frequency of recombination varied substantially, with relative hot and cold spots occurring along the whole chromosome. Eight repeated DNA sequence families were found in a complex arrangement across the centromeric region and nowhere else on the chromosome.


R. Schmidt, J. West, K. Love, Z. Lenehan, C. Lister, H. Thompson, C. Dean, Department of Molecular Genetics, Biotechnology and Biological Sciences Research Council, John Innes Centre, Colney, Norwich NR4 7UH, UK.
D. Bouchez, Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France.
(1) Present address: Max-Delbrueck Laboratory, Carl-von-Linne-Weg 10, D50829, Cologne, Germany.
(2) To whom correspondence should be addressed.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)