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Science 17 November 1995:
Vol. 270. no. 5239, pp. 1210 - 1213
DOI: 10.1126/science.270.5239.1210
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Reports

Pheromone Response in Yeast: Association of Bem1p with Proteins of the MAP Kinase Cascade and Actin

Thomas Leeuw,  Anne Fourest-Lieuvin,  Cunle Wu,  Janet Chenevert (1),  Karen Clark (2),  Malcolm Whiteway,  David Y. Thomas,  Ekkehard Leberer (3)

Haploid cells of the yeast Saccharomyces cerevisiae respond to mating pheromones with polarized growth toward the mating partner. This morphological response requires the function of the cell polarity establishment protein Bem1p. Immunochemical and two-hybrid protein interaction assays revealed that Bem1p interacts with two components of the pheromone-responsive mitogen-activated protein (MAP) kinase cascade, Ste20p and Ste5p, as well as with actin. Mutants of Bem1p that are associated with defective pheromone-induced polarized morphogenesis interacted with Ste5p and actin but not with Ste20p. Thus, the association of Bem1p with Ste20p and Ste5p may contribute to the conveyance of spatial information that regulates polarized rearrangement of the actin cytoskeleton during yeast mating.


T. Leeuw, A. Fourest-Lieuvin, C. Wu, K. Clark, M. Whiteway, D. Y. Thomas, E. Leberer, Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada.
J. Chenevert, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143-0448, USA.
(1) Present address: Station Zoologique, URA 671 Centre National de la Recherche Scientifique, 06230 Villefranche-sur-mer, France.
(2) Present address: Department of Botany, University of Maryland, College Park, MD 20742-5815, USA.
(3) To whom correspondence should be addressed.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)