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Science 15 December 1995:
Vol. 270. no. 5243, pp. 1838 - 1841
DOI: 10.1126/science.270.5243.1838
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Reports
A Nucleic Acid Triple Helix Formed by a Peptide Nucleic
Acid-DNA Complex
Laurie Betts,
John A. Josey (1),
James M. Veal,
Steven R. Jordan
The crystal structure of a nucleic acid triplex reveals a helix,
designated P-form, that differs from previously reported nucleic acid
structures. The triplex consists of one polypurine DNA strand complexed
to a polypyrimidine hairpin peptide nucleic acid (PNA) and was
successfully designed to promote Watson-Crick and Hoogsteen base
pairing. The P-form helix is underwound, with a base tilt similar to
B-form DNA. The bases are displaced from the helix axis even more than
in A-form DNA. Hydrogen bonds between the DNA backbone and the
Hoogsteen PNA backbone explain the observation that polypyrimidine PNA
sequences form highly stable 21 PNA-DNA complexes. This
structure expands the number of known stable helical forms that nucleic
acids can adopt.
Glaxo Wellcome, 5 Moore Drive, Research Triangle Park, NC 27709,
USA.
(1) Present address: Amgen Boulder, 3200 Walnut Street, Boulder, CO
80301, USA.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Orientation Preferences of Backbone Secondary Amide Functional Groups in Peptide Nucleic Acid Complexes: Quantum Chemical Calculations Reveal an Intrinsic Preference of Cationic D-Amino Acid-Based Chiral PNA Analogues for the P-form.
- C. M. Topham and J. C. Smith (2007)
Biophys. J.
92, 769-786
| Abstract »
| Full Text »
| PDF »
- The PNA-DNA hybrid I-motif: implications for sugar-sugar contacts in i-motif tetramerization.
- S. Modi, A. H. Wani, and Y. Krishnan (2006)
Nucleic Acids Res.
34, 4354-4363
| Abstract »
| Full Text »
| PDF »
- Sequence-Selective Recognition of Extended-Spectrum {beta}-Lactamase GES-2 by a Competitive, Peptide Nucleic Acid-Based Multiplex PCR Assay.
- G. F. Weldhagen (2004)
Antimicrob. Agents Chemother.
48, 3402-3406
| Abstract »
| Full Text »
| PDF »
- Insights into peptide nucleic acid (PNA) structural features: The crystal structure of a D-lysine-based chiral PNA-DNA duplex.
- V. Menchise, G. De Simone, T. Tedeschi, R. Corradini, S. Sforza, R. Marchelli, D. Capasso, M. Saviano, and C. Pedone (2003)
PNAS
100, 12021-12026
| Abstract »
| Full Text »
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- Transcription Factor Decoy Molecules Based on a Peptide Nucleic Acid (PNA)-DNA Chimera Mimicking Sp1 Binding Sites.
- M. Borgatti, I. Lampronti, A. Romanelli, C. Pedone, M. Saviano, N. Bianchi, C. Mischiati, and R. Gambari (2003)
J. Biol. Chem.
278, 7500-7509
| Abstract »
| Full Text »
| PDF »
- DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes.
- B. S. Gaylord, A. J. Heeger, and G. C. Bazan (2002)
PNAS
99, 10954-10957
| Abstract »
| Full Text »
| PDF »
- Affinity Purification of DNA and RNA from Environmental Samples with Peptide Nucleic Acid Clamps.
- D. P. Chandler, J. R. Stults, S. Cebula, B. L. Schuck, D. W. Weaver, K. K. Anderson, M. Egholm, and F. J. Brockman (2000)
Appl. Envir. Microbiol.
66, 3438-3445
| Abstract »
| Full Text »
- Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future.
- A. RAY and B. NORDÉN (2000)
FASEB J
14, 1041-1060
| Abstract »
| Full Text »
- Interaction of the Human NF-kappa B p52 Transcription Factor with DNA-PNA Hybrids Mimicking the NF-kappa B Binding Sites of the Human Immunodeficiency Virus Type 1 Promoter.
- C. Mischiati, M. Borgatti, N. Bianchi, C. Rutigliano, M. Tomassetti, G. Feriotto, and R. Gambari (1999)
J. Biol. Chem.
274, 33114-33122
| Abstract »
| Full Text »
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- PD-loop: A complex of duplex DNA with an oligonucleotide.
- N. O. Bukanov, V. V. Demidov, P. E. Nielsen, and M. D. Frank-Kamenetskii (1998)
PNAS
95, 5516-5520
| Abstract »
| Full Text »
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- Parallel and Antiparallel (G·GC)2 Triple Helix Fragments in a Crystal Structure.
- D. Vlieghe, L. Van Meervelt, A. Dautant, B. Gallois, G. Précigoux, and O. Kennard (1996)
Science
273, 1702-1705
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