Related Content
Search Google Scholar for:
More Information
Related Jobs from ScienceCareers
|
|
Science 29 November 1996:
Vol. 274. no. 5292, pp. 1511 - 1514
DOI: 10.1126/science.274.5292.1511
|
|
Reports
Essential Yeast Protein with Unexpected Similarity to Subunits of
Mammalian Cleavage and Polyadenylation Specificity Factor (CPSF)
Guillaume Chanfreau,
*
Suzanne M. Noble,
*
Christine Guthrie
The 3 ends of most eukaryotic messenger RNAs are generated by
internal cleavage and polyadenylation. In mammals, there is a strict
dependence of both reactions on the sequence AAUAAA, which occurs
upstream of polyadenylation [poly(A)] sites and which is recognized
by CPSF. In contrast, cis-acting signals for yeast 3-end generation
are highly divergent from those of mammals, suggesting that
trans-acting factors other than poly(A) polymerase would not be
conserved. The essential yeast protein Brr5/Ysh1 shows sequence
similarity to subunits of mammalian CPSF and is required for 3-end
processing in vivo and in vitro. These results demonstrate a structural
and functional conservation of the yeast and mammalian 3-end
processing machineries despite a lack of conservation of the cis
sequences.
Department of Biochemistry and Biophysics, UCSF School of
Medicine, San Francisco, CA 94143-0448. E-mail:
guthrie{at}cgl.ucsf.edu
*
Contributed equally to this work.
To whom correspondence should be addressed.
Read the Full Text
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis.
- M. Garas, B. Dichtl, and W. Keller (2008)
RNA
14, 2671-2684
| Abstract »
| Full Text »
| PDF »
- The hSNM1 protein is a DNA 5'-exonuclease.
- J. Hejna, S. Philip, J. Ott, C. Faulkner, and R. Moses (2007)
Nucleic Acids Res.
35, 6115-6123
| Abstract »
| Full Text »
| PDF »
- The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae.
- A. ZHELKOVSKY, Y. TACAHASHI, T. NASSER, X. HE, U. STERZER, T. H. JENSEN, H. DOMDEY, and C. MOORE (2006)
RNA
12, 435-445
| Abstract »
| Full Text »
| PDF »
- Human Immunodeficiency Virus Type 1 Tat Increases the Expression of Cleavage and Polyadenylation Specificity Factor 73-Kilodalton Subunit Modulating Cellular and Viral Expression.
- M. A. Calzado, R. Sancho, and E. Munoz (2004)
J. Virol.
78, 6846-6854
| Abstract »
| Full Text »
| PDF »
- Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease.
- K. RYAN, O. CALVO, and J. L. MANLEY (2004)
RNA
10, 565-573
| Abstract »
| Full Text »
| PDF »
- Functional dissection of the zinc finger and flanking domains of the Yth1 cleavage/polyadenylation factor.
- Y. Tacahashi, S. Helmling, and C. L. Moore (2003)
Nucleic Acids Res.
31, 1744-1752
| Abstract »
| Full Text »
| PDF »
- A novel transgenic line of mice exhibiting autosomal recessive male-specific lethality and non-alcoholic fatty liver disease.
- V. E. Sollars, B. J. McEntee, J. B. Engiles, J. L. Rothstein, and A. M. Buchberg (2002)
Hum. Mol. Genet.
11, 2777-2786
| Abstract »
| Full Text »
| PDF »
- Metallo-{beta}-lactamase fold within nucleic acids processing enzymes: the {beta}-CASP family.
- I. Callebaut, D. Moshous, J.-P. Mornon, and J.-P. de Villartay (2002)
Nucleic Acids Res.
30, 3592-3601
| Abstract »
| Full Text »
| PDF »
- Comparative genomics and evolution of proteins involved in RNA metabolism.
- V. Anantharaman, E. V. Koonin, and L. Aravind (2002)
Nucleic Acids Res.
30, 1427-1464
| Abstract »
| Full Text »
| PDF »
- In silico detection of control signals: mRNA 3'-end-processing sequences in diverse species.
- J. H. Graber, C. R. Cantor, S. C. Mohr, and T. F. Smith (1999)
PNAS
96, 14055-14060
| Abstract »
| Full Text »
| PDF »
- Pta1, a Component of Yeast CF II, Is Required for Both Cleavage and Poly(A) Addition of mRNA Precursor.
- J. Zhao, M. Kessler, S. Helmling, J. P. O'Connor, and C. Moore (1999)
Mol. Cell. Biol.
19, 7733-7740
| Abstract »
| Full Text »
| PDF »
- The Cleavage and Polyadenylation Specificity Factor in Xenopus laevis Oocytes Is a Cytoplasmic Factor Involved in Regulated Polyadenylation.
- K. S. Dickson, A. Bilger, S. Ballantyne, and M. P. Wickens (1999)
Mol. Cell. Biol.
19, 5707-5717
| Abstract »
| Full Text »
| PDF »
- Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis.
- J. Zhao, L. Hyman, and C. Moore (1999)
Microbiol. Mol. Biol. Rev.
63, 405-445
| Abstract »
| Full Text »
| PDF »
- Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast.
- J. B. McNeil, H. Agah, and D. Bentley (1998)
Genes & Dev.
12, 2510-2521
| Abstract »
| Full Text »
- Mechanism and regulation of mRNA polyadenylation.
- D. F. Colgan and J. L. Manley (1997)
Genes & Dev.
11, 2755-2766
| Full Text »
| PDF »
- A Genomic Perspective on Protein Families.
- R. L. Tatusov, E. V. Koonin, and D. J. Lipman (1997)
Science
278, 631-637
| Abstract »
| Full Text »
- Alternative 3'-end processing of U5 snRNA by RNase III.
- G. Chanfreau, S. A. Elela, M. Ares Jr., and C. Guthrie (1997)
Genes & Dev.
11, 2741-2751
| Abstract »
| Full Text »
| PDF »
- Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast.
- M. M. Kessler, M. F. Henry, E. Shen, J. Zhao, S. Gross, P. A. Silver, and C. L. Moore (1997)
Genes & Dev.
11, 2545-2556
| Abstract »
| Full Text »
| PDF »
- The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins..
- S M Barabino, W Hubner, A Jenny, L Minvielle-Sebastia, and W Keller (1997)
Genes & Dev.
11, 1703-1716
| Abstract »
| PDF »
- Cleavage Factor II of Saccharomyces cerevisiae Contains Homologues to Subunits of the Mammalian Cleavage/ Polyadenylation Specificity Factor and Exhibits Sequence-specific, ATP-dependent Interaction with Precursor RNA.
- J. Zhao, M. M. Kessler, and C. L. Moore (1997)
J. Biol. Chem.
272, 10831-10838
| Abstract »
| Full Text »
| PDF »
|
|
