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Science 19 December 1997:
Vol. 278. no. 5346, pp. 2130 - 2133
DOI: 10.1126/science.278.5346.2130
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Reports

Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts

Angelika E. Schnieke, * Alexander J. Kind, William A. Ritchie, Karen Mycock, Angela R. Scott, Marjorie Ritchie, Ian Wilmut, Alan Colman, Keith H. S. Campbell dagger

Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.

A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK.
W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK.
*   To whom correspondence should be addressed.

dagger    Present address: PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)