Asymmetric Tethering of Flat and Curved Lipid Membranes by a Golgin
Guillaume Drin,1
Vincent Morello,1
Jean-François Casella,1
Pierre Gounon,2
Bruno Antonny1*
Golgins, long stringlike proteins, tether cisternae and transport vesicles at the Golgi apparatus. We examined the attachment of golgin GMAP-210 to lipid membranes. GMAP-210 connected highly curved liposomes to flatter ones. This asymmetric tethering relied on motifs that sensed membrane curvature both in the N terminus of GMAP-210 and in ArfGAP1, which controlled the interaction of the C terminus of GMAP-210 with the small guanine nucleotide–binding protein Arf1. Because membrane curvature constantly changes during vesicular trafficking, this mode of tethering suggests a way to maintain the Golgi architecture without compromising membrane flow.
1 Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia Antipolis and CNRS, 660 route des lucioles, 06560 Valbonne, France.
2 Centre Commun de Microscopie Appliquée, Parc Valrose, 06108 Nice Cedex 2, France.
* To whom correspondence should be addressed. E-mail: antonny{at}ipmc.cnrs.fr
