Modulation of Blood Fluke Development in the Liver by Hepatic CD4+ Lymphocytes Stephen J. Davies, Jane L. Grogan, Rebecca B. Blank, K. C. Lim, Richard M. Locksley, and James H. McKerrow

Supplementary Material

Supplemental Figure 1. Perivascular accumulations of lymphoid cells associate with developing parasites in wild-type mice, but not in RAG1^-/- mice. A perivascular infiltrate containing lymphocytes (identified as possessing small, dark-staining nuclei; two examples are highlighted by arrows) is evident around parasites in wild type mice. No cellular infiltrate is evident around parasites in RAG1^-/- mice. Samples of liver tissue from infected wild type C57BL/6 and RAG1^-/- mice were obtained at day 19 p.i., fixed in 4% formaldehyde, embedded in paraffin, sectioned to 5 m and stained with hematoxylin and eosin. Two representative micrographs from each host genotype are shown. 400x magnification.

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Supplemental Figure 2. CD4⁺ TCR⁺ cells from the livers of 2-m^-/-/MHCII^-/- mice display lymphoid morphology. Liver leukocytes were isolated from the livers of 2-m^-/-/MHCII^-/- mice, and stained for CD4, TCR and CD19. CD4⁺ TCR⁺ double-positive cells were sorted to high purity by high-throughput FACS, spun onto slides and stained with DiffQuick stain. 1000x magnification.

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