Synaptotagmin Modulation of Fusion Pore Kinetics in Regulated Exocytosis of Dense-Core Vesicles Chih-Tien Wang, Ruslan Grishanin, Cynthia A. Earles, Payne Y. Chang, Thomas F. J. Martin, Edwin R. Chapman, and Meyer B. Jackson

Supplementary Material

Supplemental Figure 1. A. and B. Comparison of spike height and quantal size for control cells and cells transfected with proteins. The mean spike height and quantal size (number of molecules) were not changed by synaptotagmin I or IV, but transfection with 1A (P/Q-type) Ca⁺² channels increased these quantities significantly (** p < 0.01; *** p < 0.001). C. The distribution of the cube root of quantal size (spike area) was unchanged by synaptotagmin I and IV, but was shifted to the right by 1A Ca⁺² channels (p < 0.05). All the changes with Ca⁺² channels are consistent with a slight excess of large vesicles, possibly due to vesicle-vesicle fusion events prior to exocytosis (1, 2).

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