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Published Online May 15, 2008
Science DOI: 10.1126/science.1159151

Research Articles

Submitted on April 15, 2008
Accepted on May 1, 2008

Widespread Translational Inhibition by Plant miRNAs and siRNAs

Peter Brodersen 1, Lali Sakvarelidze-Achard 1, Marianne Bruun-Rasmussen 1, Patrice Dunoyer 1, Yoshiharu Y. Yamamoto 2, Leslie Sieburth 3, Olivier Voinnet 1*

1 Institut de Biologie Moléculaire des Plantes du CNRS, UPR2357; 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France.
2 Center for Gene Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya Aichi, 464-8602, Japan.
3 Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.

* To whom correspondence should be addressed.
Olivier Voinnet , E-mail: olivier.voinnet{at}ibmp-ulp.u-strasbg.fr

High complementarity between plant miRNAs and their mRNA targets is thought to cause silencing prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by short interfering (si)RNA populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action as recently suggested from animal studies. Also as in animals, the decapping component VCS/Ge-1 is required for translational repression by miRNAs, suggesting that the underlying mechanisms in the two kingdoms are related.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)