Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 3 August 1990:
Vol. 249. no. 4968, pp. 511 - 517
DOI: 10.1126/science.2166334
Prev | Table of Contents | Next

Articles

Science, Vol 249, Issue 4968, 511-517
Copyright © 1990 by American Association for the Advancement of Science

articles

The Hin invertasome: protein-mediated joining of distant recombination sites at the enhancer

KA Heichman and RC Johnson

Department of Biological Chemistry, UCLA School of Medicine.

The Hin protein binds to two cis-acting recombination sites and catalyzes a site-specific DNA inversion reaction that regulates the expression of flagellin genes in Salmonella. In addition to the Hin protein and the two recombination sites that flank the invertible segment, a third cis-acting recombinational enhancer sequence and the Fis protein, which binds to two sites within the enhancer, are required for efficient recombination. Intermediates of this reaction were trapped during DNA strand cleavage and analyzed by gel electrophoresis and electron microscopy in order to determine their structure and composition. The analyses demonstrate that the recombination sites are assembled at the enhancer into a complex nucleo-protein structure (termed the invertasome) with the looping of the three segments of intervening DNA. Antibody studies indicated that Fis physically interacts with Hin and that both proteins are intimately associated with the invertasome. In order to achieve this protein-protein interaction and assemble the invertasome, the substrate DNA must be supercoiled.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Common and Variable Contributions of Fis Residues to High-Affinity Binding at Different DNA Sequences.
L. S. Feldman-Cohen, Y. Shao, D. Meinhold, C. Miller, W. Colon, and R. Osuna (2006)
J. Bacteriol. 188, 2081-2095
   Abstract »    Full Text »    PDF »
Two DNA Invertases Contribute to Flagellar Phase Variation in Salmonella enterica Serovar Typhimurium Strain LT2.
K. Kutsukake, H. Nakashima, A. Tominaga, and T. Abo (2006)
J. Bacteriol. 188, 950-957
   Abstract »    Full Text »    PDF »
FljA-Mediated Posttranscriptional Control of Phase 1 Flagellin Expression in Flagellar Phase Variation of Salmonella enterica Serovar Typhimurium.
S. Yamamoto and K. Kutsukake (2006)
J. Bacteriol. 188, 958-967
   Abstract »    Full Text »    PDF »
Phase and Antigenic Variation in Bacteria.
M. W. van der Woude and A. J. Baumler (2004)
Clin. Microbiol. Rev. 17, 581-611
   Abstract »    Full Text »    PDF »
A global role for Fis in the transcriptional control of metabolism and type III secretion in Salmonella enterica serovar Typhimurium.
A. Kelly, M. D. Goldberg, R. K. Carroll, V. Danino, J. C. D. Hinton, and C. J. Dorman (2004)
Microbiology 150, 2037-2053
   Abstract »    Full Text »    PDF »
Effect of DNA Superhelicity and Bound Proteins on Mechanistic Aspects of the Hin-Mediated and Fis-Enhanced Inversion.
J. Huang, Q. Zhang, and T. Schlick (2003)
Biophys. J. 85, 804-817
   Abstract »    Full Text »    PDF »
Involvement of a Bifunctional, Paired-like DNA-binding Domain and a Transpositional Enhancer in Sleeping Beauty Transposition.
Z. Izsvak, D. Khare, J. Behlke, U. Heinemann, R. H. Plasterk, and Z. Ivics (2002)
J. Biol. Chem. 277, 34581-34588
   Abstract »    Full Text »    PDF »
Flagellar Phase Variation of Salmonella enterica Serovar Typhimurium Contributes to Virulence in the Murine Typhoid Infection Model but Does Not Influence Salmonella-Induced Enteropathogenesis.
J. S. Ikeda, C. K. Schmitt, S. C. Darnell, P. R. Watson, J. Bispham, T. S. Wallis, D. L. Weinstein, E. S. Metcalf, P. Adams, C. D. O'Connor, et al. (2001)
Infect. Immun. 69, 3021-3030
   Abstract »    Full Text »    PDF »
Topoisomerase IV, alone, unknots DNA in E. coli.
R. W. Deibler, S. Rahmati, and E. L. Zechiedrich (2001)
Genes & Dev. 15, 748-761
   Abstract »    Full Text »
Hin Recombinase Mutants Functionally Disrupted in Interactions with Fis.
O. Z. Nanassy and K. T. Hughes (2001)
J. Bacteriol. 183, 28-35
   Abstract »    Full Text »
Multiple Gene Products and Sequences Required for Excision of the Mobilizable Integrated Bacteroides Element NBU1.
N. B. Shoemaker, G.-R. Wang, and A. A. Salyers (2000)
J. Bacteriol. 182, 928-936
   Abstract »    Full Text »
RAG-2 Promotes Heptamer Occupancy by RAG-1 in the Assembly of a V(D)J Initiation Complex.
P. C. Swanson and S. Desiderio (1999)
Mol. Cell. Biol. 19, 3674-3683
   Abstract »    Full Text »    PDF »
In Vivo Assay of Protein-Protein Interactions in Hin-Mediated DNA Inversion.
S. Y. Lee, H. J. Lee, H. Lee, S. Kim, E. H. Cho, and H. M. Lim (1998)
J. Bacteriol. 180, 5954-5960
   Abstract »    Full Text »
Communication between Hin recombinase and Fis regulatory subunits during coordinate activation of Hin-catalyzed site-specific DNA inversion.
S. K. Merickel, M. J. Haykinson, and R. C. Johnson (1998)
Genes & Dev. 12, 2803-2816
   Abstract »    Full Text »
In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catalyzed by the Salmonella Hin Recombinase.
O. Z. Nanassy and K. T. Hughes (1998)
Genetics 149, 1649-1663
   Abstract »    Full Text »    PDF »
Modulation of Activity of Moloney Murine Leukemia Virus Preintegration Complexes by Host Factors In Vitro.
L. Li, C. M. Farnet, W. F. Anderson, and F. D. Bushman (1998)
J. Virol. 72, 2125-2131
   Abstract »    Full Text »    PDF »
Hin-mediated Inversion on Positively Supercoiled DNA.
H. M. Lim, H. J. Lee, C. Jaxel, and M. Nadal (1997)
J. Biol. Chem. 272, 18434-18439
   Abstract »    Full Text »    PDF »
Enhancer-independent variants of phage Mu transposase: enhancer-specific stimulation of catalytic activity by a partner transposase..
J Y Yang, M Jayaram, and R M Harshey (1995)
Genes & Dev. 9, 2545-2555
   Abstract »    PDF »
Circular Structures in Retroviral and Cellular Genomes.
F. G. Albert, E. C. Bronson, D. J. Fitzgerald, and J. N. Anderson (1995)
J. Biol. Chem. 270, 23570-23581
   Abstract »    Full Text »    PDF »
Hin recombinase bound to DNA: the origin of specificity in major and minor groove interactions.
J. Feng, R. Johnson, and R. Dickerson (1994)
Science 263, 348-355
   Abstract »    PDF »
Site-specific HU binding in the Mu transpososome: conversion of a sequence-independent DNA-binding protein into a chemical nuclease..
B D Lavoie and G Chaconas (1993)
Genes & Dev. 7, 2510-2519
   Abstract »    PDF »
Analysis of a nucleoprotein complex: the synaptosome of gamma delta resolvase.
N. Grindley (1993)
Science 262, 738-740
   Abstract »    PDF »
The nonspecific DNA-binding and -bending proteins HMG1 and HMG2 promote the assembly of complex nucleoprotein structures..
T T Paull, M J Haykinson, and R C Johnson (1993)
Genes & Dev. 7, 1521-1534
   Abstract »    PDF »
Role of Gin and FIS in Site-specific Recombination.
P. Merker, G. Muskhelishvili, A. Deufel, K. Rusch, and R. Kahmann (1993)
Cold Spring Harb Symp Quant Biol 58, 505-513
   Abstract »    PDF »
Configuration of DNA strands and mechanism of strand exchange in the Hin invertasome as revealed by analysis of recombinant knots..
K A Heichman, I P Moskowitz, and R C Johnson (1991)
Genes & Dev. 5, 1622-1634
   Abstract »    PDF »
Alignment of recombination sites in Hin-mediated site-specific DNA recombination..
I P Moskowitz, K A Heichman, and R C Johnson (1991)
Genes & Dev. 5, 1635-1645
   Abstract »    PDF »
Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection.
T. Blackwell and H Weintraub (1990)
Science 250, 1104-1110
   Abstract »    PDF »
Sequence-specific DNA binding by the c-Myc protein.
T. Blackwell, L Kretzner, E. Blackwood, R. Eisenman, and H Weintraub (1990)
Science 250, 1149-1151
   Abstract »    PDF »
The Mu Enhancer Is Functionally Asymmetric Both in cis and in trans. TOPOLOGICAL SELECTIVITY OF Mu TRANSPOSITION IS ENHANCER-INDEPENDENT.
H. Jiang and R. M. Harshey (2001)
J. Biol. Chem. 276, 4373-4381
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)