Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Applied Bio SDS

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 May 2008:
Vol. 320. no. 5877, pp. 797 - 799
DOI: 10.1126/science.1152095
Prev | Table of Contents | Next

Reports

Phosphorylation of Retinoblastoma Protein by Viral Protein with Cyclin-Dependent Kinase Function

Adam J. Hume,1 Jonathan S. Finkel,2 Jeremy P. Kamil,3 Donald M. Coen,3 Michael R. Culbertson,2 Robert F. Kalejta1*

As obligate intracellular parasites, viruses expertly modify cellular processes to facilitate their replication and spread, often by encoding genes that mimic the functions of cellular proteins while lacking regulatory features that modify their activity. We show that the human cytomegalovirus UL97 protein has activities similar to cellular cyclin–cyclin-dependent kinase (CDK) complexes. UL97 phosphorylated and inactivated the retinoblastoma tumor suppressor, stimulated cell cycle progression in mammalian cells, and rescued proliferation of Saccharomyces cerevisiae lacking CDK activity. UL97 is not inhibited by the CDK inhibitor p21 and lacks amino acid residues conserved in the CDKs that permit the attenuation of kinase activity. Thus, UL97 represents a functional ortholog of cellular CDKs that is immune from normal CDK control mechanisms.

1 Institute for Molecular Virology and McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA.
2 Laboratories of Genetics and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706, USA.
3 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

* To whom correspondence should be addressed. E-mail: rfkalejta{at}wisc.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cell Cycle-Independent Expression of Immediate-Early Gene 3 Results in G1 and G2 Arrest in Murine Cytomegalovirus-Infected Cells.
L. Wiebusch, A. Neuwirth, L. Grabenhenrich, S. Voigt, and C. Hagemeier (2008)
J. Virol. 82, 10188-10198
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)