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Science 8 November 1996:
Vol. 274. no. 5289, pp. 893 - 0
DOI: 10.1126/science.274.5289.893e
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This Week in Science
Imaging single molecules often requires immobilizing the molecules in solids or at surfaces or at low temperatures to slow down molecular motion. Application of such methods to biological molecules, however, would require imaging in aqueous solution if the results are to be meaningful. Dickson et al. (p. 966) used the water-filled pores present in a polyacrylamide gel, whose pore size can be tuned by changing the concentration of the gel, to restrict the Brownian motion of molecules. Laser excitation was performed in a thin plane so that the lateral motion of a molecule could be followed for about 1 second. For example, a dye molecule, nile red, could be imaged in a gel with 2-nanometer pores. Larger molecules, such as fluorescently tagged antibodies, were imaged in the same manner by using less concentrated gels with larger pores.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)
